Alexa Fluor(R) 488 Rat IgG2b, kappa Isotype Ctrl 400625 from BioLegend

Product Specs
ItemAlexa Fluor(R) 488 Rat IgG2b, kappa Isotype Ctrl
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number400625
IsotypeIgG2b, Kappa
HostRat
ApplicationsFlow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC)
Conjugate/TagAlexa Fluor® 488
Quantity100 µg
CloneRTK4530
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenTrinitrophenol + KLH
ClonalityMonoclonal

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Improving Cancer Targeting: A Study on the Effect of Dual-Ligand Density on Targeting of Cells Having Differential Expression of Target BiomarkersInternational Journal of Molecular SciencesAugust 22, 2023Nayer Sultana, Allan E. Davidconjugated Rat (IgG2b,k) monoclonal antibody (Clone IM7, Cat#103015), isotype controls (Clone RTK4530, Cat#400625), zombie violet dye, fixation buffer were purchased from BioLegend. Alexa Fluor 647 conjugated Mouse

(2) The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymiseLifeDecember 14, 2022Pleuger C, Ai D, Hoppe ML, Winter LT, Bohnert D, Karl D, Guenther S, Epelman S, Kantores C, Fijak M, et al.1:200 Isotype control Antibody,"Alexa Fluor 488 Rat IgG2b, κ Isotype Ctrl antibody",BioLegend,Cat.No.: 400625; RRID: AB_389321,FC 1:200 Isotype control Antibody,"PE Isotype Control Antibody, Rat IgG2a",Miltenyi,Cat.No.:

(3) Expression of membrane Hsp90 is a molecular signature of T cell activationScientific ReportsOctober 27, 2022Scott A. Scarneo, Aaron P. Smith, Jacob Favret, Robert Oâ€™Connell, Joy Pickeral, Kelly W. Yang, Guido Ferrari, David R. Loiselle, Philip F. Hughes, Manjusha M. Kulkarni, et al.,BD Biosciences,1:1500 103121,CD45-AF488,BioLegend,1:1000 300454,CD3-AF488,BioLegend,1:1000 301817,CD14-AF488,BioLegend,1:1000 400625,Rat IgG2b Isotype Control,BioLegend,1:1000 Flow cytometry antibodies.

(4) C3G down-regulation enhances pro-migratory and stemness properties of oval cells by promoting an epithelial-mesenchymal-like processInternational Journal of Biological SciencesSeptember 25, 2022Nerea Palao, Celia Sequera, Ãngel M Cuesta, Cristina Baquero, Paloma Bragado, Alvaro Gutierrez-Uzquiza, ArÃ¡nzazu SÃ¡nchez, Carmen Guerrero, Almudena Porrasincubated with CD44-A488 antibody (1:50, Biolegend#103016) or control rat isotype IgG (1:50, Biolegend#400625) in 0.1% BSA-PBS. Fluorescence intensity was analyzed using a FACSCalibur flow cytometer. Induction

(5) Disruption of the endopeptidase ADAM10-Notch signaling axis leads to skin dysbiosis and innate lymphoid cell-mediated hair follicle destructionImmunityOctober 12, 2021Sakamoto K, Jin SP, Goel S, Jo JH, Voisin B, Kim D, Nadella V, Liang H, Kobayashi T, Huang X, et al.(RTK2758)",Biolegend,Cat# 400502; RRID: AB_326523 "AF488 rat IgG2α,κ isotype Ctrl (RTK4530)",Biolegend,Cat# 400625; RRID: AB_389321 "AF647 rat IgG2 β,κ Isotype Ctrl (RTK4530)",Biolegend,Cat# 400626; RRID: AB_389343 Alexa Fluor™ 488 Antibody Labeling Kit

(6) Mutations in topoisomerase IIβ result in a B cell immunodeficiencyNature communicationsAugust 13, 2019Broderick L, Yost S, Li D, McGeough MD, Booshehri LM, Guaderrama M, Brydges SD, Kucharova K, Patel NC, Harr M, et al.BioLegend, Inc., 142408). The following isotype controls: (1) Alexa Fluor 488 Rat IgG2b, kappa (BioLegend, Inc., 400625), (2) eFluor 570 Rat IgG2a, kappa (ThermoFisher Scientific, Inc., 41-4321-82), and (3) Alexa Fluor 647

(7) Alginate encapsulation as long-term immune protection of allogeneic pancreatic islet cells transplanted into the omental bursa of macaquesNature biomedical engineeringNovember 1, 2018Bochenek MA, Veiseh O, Vegas AJ, McGarrigle JJ, Qi M, Marchese E, Omami M, Doloff JC, Mendoza-Elias J, Nourmohammadzadeh M, et al.and laser intensity settings, unstained, single antibody, and IgG (labeled with either Alexa-488 (CA# 400625, BioLegend) or Alexa-647 (CA# 400526, BioLegend) controls were also run. Histology of retrieved freely

(8) Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In VivoCell stem cellApril 5, 2018Kooreman NG, Kim Y, de Almeida PE, Termglinchan V, Diecke S, Shao NY, Wei TT, Yi H, Dey D, Nelakanti R, et al.Rat IgG2b κ isotype control,Biolegend,400627 Alexa Fluor 488 Rat IgG2b κ isotype control,Biolegend,400625 PE Rat IgG2b κ isotype control,Thermo-Fisher Scientific,12-4031-82; RRID: AB_470042 FITC Rat IgG2a κ isotype control

(9) Loss of NF-κB1 Causes Gastric Cancer with Aberrant Inflammation and Expression of Immune Checkpoint Regulators in a STAT-1-Dependent MannerImmunityMarch 20, 2018O'Reilly LA, Putoczki TL, Mielke LA, Low JT, Lin A, Preaudet A, Herold MJ, Yaprianto K, Tai L, Kueh A, et al.

(10) 4-Methylumbelliferone Suppresses Hyaluronan Synthesis and Tumor Progression in SCID Mice Intra-abdominally Inoculated With Pancreatic Cancer CellsPancreasFebruary 1, 2017Hayato Nagase, Daisuke Kudo, Akiko Suto, Eri Yoshida, Shinichiro Suto, Mika Negishi, Ikuko Kakizaki, Kenichi Hakamadaanti–mouse/human CD44 antibody (cat. no. 103016; BioLegend, San Diego, Calif) or an isotype control (cat. no. 400625; BioLegend) for 30 minutes at 4°C. In both assays, the cells were harvested using Cell Dissociation

(11) STAT2 Is a Pervasive Cytokine Regulator due to Its Inhibition of STAT1 in Multiple Signaling PathwaysPLoS biologyOctober 1, 2016Ho J, Pelzel C, Begitt A, Mee M, Elsheikha HM, Scott DJ, Vinkemeier U.rat antimouse IA/I-E (#107615, Biolegend) or 4 μg/ml Alexa Fluor-488 rat IgG2b, κ isotype control (#400625, Biolegend) for the detection of MHC class II expression on mouse macrophages. MHC class I on human

(12) Combinatorial hydrogel library enables identification of materials that mitigate the foreign body response in primatesNature biotechnologyMarch 1, 2016Vegas AJ, Veiseh O, Doloff JC, Ma M, Tam HH, Bratlie K, Li J, Bader AR, Langan E, Olejnik K, et al.and laser intensity settings, unstained, single antibody, and IgG (labeled with either Alexa-488 (CA# 400625)35 or Alexa-647 (CA# 400526, BioLegend)35 controls were also run. Intravital imaging For intravital imaging

(13) Basal body multipotency and axonemal remodelling are two pathways to a 9+0 flagellumNature communicationsDecember 15, 2015Wheeler RJ, Gluenz E, Gull K.

(14) The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastomaSpringerPlusJanuary 1, 2015Santhana Kumar K, Tripolitsioti D, Ma M, Grählert J, Egli KB, Fiaschetti G, Shalaby T, Grotzer MA, Baumgartner M.stained with the following primary antibodies: CD44-Alexa488 (103016, 1:50), Isotype control-Alexa488 (400625, 1:50) (BioLegend), c-Met-biotin (13–8858, 1:100), c-Met (5631, 1:100) (Cell Signaling), Isotype control-biotin

(15) Spdef null mice lack conjunctival goblet cells and provide a model of dry eyeThe American journal of pathologyJuly 1, 2013Marko CK, Menon BB, Chen G, Whitsett JA, Clevers H, Gipson IK.

(16) ICOS promotes IL-17 synthesis in colonic intraepithelial lymphocytes in IL-10-/- miceJournal of leukocyte biologyFebruary 1, 2010Schaefer JS, Montufar-Solis D, Vigneswaran N, Klein JR.

(17) Pattern recognition scavenger receptor SRA/CD204 down-regulates Toll-like receptor 4 signaling-dependent CD8 T-cell activationBloodJune 4, 2009Yi H, Yu X, Gao P, Wang Y, Baek SH, Chen X, Kim HL, Subjeck JR, Wang XY.

(18) Microbial carbohydrate depolymerization by antigen-presenting cells: deamination prior to presentation by the MHCII pathwayProceedings of the National Academy of Sciences of the United States of AmericaApril 1, 2008Duan J, Avci FY, Kasper DL.

(19) The nature of the allosteric interactions of ribonuclease and its ligandsThe Biochemical journalJuly 1, 1978E J Walker, G B Ralston, I G Darvey

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(1)

9/12/2016Great Flow Cytometry Isotype Control Andy GonzalezI use flow cytometry to determine what immune cells respond during viral infection in mice. I chose this antibody because it was a recommended isotype control for two other antibodies I use.

Alexa Fluor(R) 488 Rat IgG2b, kappa Isotype Ctrl from BioLegend

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Alexa Fluor(R) 488 Rat IgG2b, kappa Isotype Ctrl from BioLegend

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